Wednesday, August 11, 2010

New microscope at IU Bloomington breaks light microscopy resolution barrier

A fixed PTK (marsupial kidney cell line) cell in mitosis. The condensed chromosomes are stained with Hoechst stain and are shown in blue, while microtubules are labelled with an antibody to tubulin and are shown in red. Imaging was done in the structured illumination mode. More than 2000 images were taken and processed to create this image.2010Image by Eric Workman and Jim PowersFixed PTK cell in mitosis.
Image by Eric Workman and Jim Powers  

BLOOMINGTON, Ind. -- A new laser-equipped microscope at IU Bloomington's Light Microscopy Imaging Center makes it possible to examine biological samples with unprecedented detail in three dimensions.

A fixed PTK (marsupial kidney cell line) cell in mitosis. The condensed chromosomes are stained with Hoechst stain and are shown in blue, while microtubules are labelled with an antibody to tubulin and are shown in red. Imaging was done in the structured illumination mode. More than 2000 images were taken and processed to create this image.

...
"It's a fantastic and unique acquisition for our university," said cell biologist Claire Walczak, the Imaging Center's executive director. "This super-resolution microscope, one of only 16 in the world and one of only 8 commercial units, is part of our vision to bring state-of-the-art technology to IU's life science researchers, to enable them to address questions that they did not have the ability to ask previously, due to the lack of appropriate technologies."

...
A short video of a live HeLa cell can be viewed at http://newsinfo.iu.edu/asset/page/normal/9660.html. 


Read the full article >>